Uracil-DNA glycosylase inhibitor

Details

Name
Uracil-DNA glycosylase inhibitor
Kind
protein
Synonyms
Not Available
Gene Name
UGI
UniProtKB Entry
P14739Swiss-Prot
Organism
Bacillus phage PBS2
NCBI Taxonomy ID
10684
Amino acid sequence
>lcl|BSEQ0012146|Uracil-DNA glycosylase inhibitor
MTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTAYDESTDENVMLLTS
DAPEYKPWALVIQDSNGENKIKML
Number of residues
84
Molecular Weight
9475.645
Theoretical pI
3.87
GO Classification
Not Available
General Function
This protein binds specifically and reversibly to the host uracil-DNA glycosylase, preventing removal of uracil residues from PBS2 DNA by the host uracil-excision repair system.
Specific Function
Not Available
Pfam Domain Function
Not Available
Signal Regions
Not Available
Transmembrane Regions
Not Available
Cellular Location
Not Available
Gene sequence
>lcl|BSEQ0005983|255 bp
ATGACAAATTTATCTGACATCATTGAAAAAGAAACAGGAAAACAACTAGTGATTCAAGAA
TCAATTCTAATGTTACCAGAAGAAGTAGAGGAAGTAATTGGGAATAAACCAGAAAGTGAT
ATTTTAGTTCATACTGCTTATGATGAAAGTACAGATGAAAATGTAATGCTATTAACTTCA
GATGCTCCAGAATATAAACCTTGGGCTTTAGTAATTCAAGACAGTAATGGAGAAAATAAA
ATTAAAATGTTATAA
Chromosome Location
Not Available
Locus
Not Available
External Identifiers
ResourceLink
UniProtKB IDP14739
UniProtKB Entry NameUNGI_BPPB2
GenBank Gene IDJ04434
PDB ID(s)1EUI, 1LQG, 1LQM, 1UDI, 1UGH, 1UGI, 1UUG, 2J8X, 2UGI, 2UUG, 2ZHX, 4LYL
General References
  1. Wang Z, Mosbaugh DW: Uracil-DNA glycosylase inhibitor gene of bacteriophage PBS2 encodes a binding protein specific for uracil-DNA glycosylase. J Biol Chem. 1989 Jan 15;264(2):1163-71. [Article]
  2. Lundquist AJ, Beger RD, Bennett SE, Bolton PH, Mosbaugh DW: Site-directed mutagenesis and characterization of uracil-DNA glycosylase inhibitor protein. Role of specific carboxylic amino acids in complex formation with Escherichia coli uracil-DNA glycosylase. J Biol Chem. 1997 Aug 22;272(34):21408-19. [Article]
  3. Ravishankar R, Bidya Sagar M, Roy S, Purnapatre K, Handa P, Varshney U, Vijayan M: X-ray analysis of a complex of Escherichia coli uracil DNA glycosylase (EcUDG) with a proteinaceous inhibitor. The structure elucidation of a prokaryotic UDG. Nucleic Acids Res. 1998 Nov 1;26(21):4880-7. [Article]
  4. Putnam CD, Shroyer MJ, Lundquist AJ, Mol CD, Arvai AS, Mosbaugh DW, Tainer JA: Protein mimicry of DNA from crystal structures of the uracil-DNA glycosylase inhibitor protein and its complex with Escherichia coli uracil-DNA glycosylase. J Mol Biol. 1999 Mar 26;287(2):331-46. [Article]

Associated Data

Drug Relations
DrugDrug groupPharmacological action?TypeActionsDetails
Imidazoleexperimental, investigationalunknowntargetDetails