Vitamin B12 transporter BtuB
Details
- Name
- Vitamin B12 transporter BtuB
- Synonyms
- bfe
- cer
- Cobalamin receptor
- dcrC
- Outer membrane cobalamin translocator
- Gene Name
- btuB
- Organism
- Escherichia coli (strain K12)
- Amino acid sequence
>lcl|BSEQ0011034|Vitamin B12 transporter BtuB MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLAPTTVVTRQDIDRWQST SVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDGVRLNLAGVSGSADLSQFP IALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEISAGWGSNSYQNYDVSTQQQL GDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFLSKTLYGALEHNFTDAWSGFVRG YGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRYNGELIKSQLITSYSHSKDYNYDPH YGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAGVDWQKQTTTPGTGYVEDGYDQRNTGI YLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQTSAGWEFIEGYRFIASYGTSYKAPNLGQ LYGFYGNPNLDPEKSKQWEGAFEGLTAGVNWRISGYRNDVSDLIDYDDHTLKYYNEGKAR IKGVEATANFDTGPLTHTVSYDYVDARNAITDTPLLRRAKQQVKYQLDWQLYDFDWGITY QYLGTRYDKDYSSYPYQTVKMGGVSLWDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQ TAGREYTLSGSYTF
- Number of residues
- 614
- Molecular Weight
- 68406.715
- Theoretical pI
- 5.11
- GO Classification
- Functionscobalamin transporter activity / metal ion binding / porin activity / receptor activityProcessescobalamin transport / ion transportComponentscell outer membrane / pore complex
- General Function
- Receptor activity
- Specific Function
- Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB. Is also a receptor for bacteriophages BF23 and C1, and for A and E colicins.
- Pfam Domain Function
- Transmembrane Regions
- 158-165 169-178 184-195 217-227 232-248 263-277 279-296 309-325 328-337 353-369 371-381 385-400 403-417 434-443 449-458 473-490 494-509 517-529 535-550 558-572 585-596 602-614
- Cellular Location
- Cell outer membrane
- Gene sequence
>lcl|BSEQ0011035|Vitamin B12 transporter BtuB (btuB) ATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCA CAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGC ACTGTGCTTGCACCAACCACCGTTGTGACCCGTCAGGATATCGACCGCTGGCAGTCGACC TCGGTCAATGATGTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCA GGTCAGCTCTCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATT GATGGCGTACGCCTGAATCTGGCGGGGGTGAGTGGTTCTGCCGACCTTAGCCAGTTCCCT ATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCC GATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATT TCAGCAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTG GGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTT GTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAA ACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTGGAGCGGCTTTGTGCGCGGC TATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTC GATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTG ATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCAT TATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACACCGTCCAGTGG GCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAG ACTACGACGCCGGGTACAGGTTATGTTGAGGATGGATATGATCAACGTAATACCGGCATC TATCTGACCGGGCTGCAACAAGTCGGCGATTTTACCTTTGAAGGCGCAGCACGCAGTGAC GATAACTCACAGTTTGGTCGTCATGGAACCTGGCAAACCAGCGCCGGTTGGGAATTCATC GAAGGTTATCGCTTCATTGCTTCCTACGGGACATCTTATAAGGCACCAAATCTGGGGCAA CTGTATGGCTTCTACGGAAATCCGAATCTGGACCCGGAGAAAAGCAAACAGTGGGAAGGC GCGTTTGAAGGCTTAACCGCTGGGGTGAACTGGCGTATTTCCGGATATCGTAACGATGTC AGTGACTTGATCGATTATGATGATCACACCCTGAAATATTACAACGAAGGGAAAGCGCGG ATTAAGGGCGTCGAGGCGACCGCCAATTTTGATACCGGACCACTGACGCATACTGTGAGT TATGATTATGTCGATGCGCGCAATGCGATTACCGACACGCCGTTGTTACGCCGTGCTAAA CAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTAT CAGTATTTAGGCACTCGCTATGATAAGGATTACTCATCTTATCCTTATCAAACCGTTAAA ATGGGCGGTGTGAGCTTGTGGGATCTTGCGGTTGCGTATCCGGTCACCTCTCACCTGACA GTTCGTGGTAAAATAGCCAACCTGTTCGACAAAGATTATGAGACAGTCTATGGCTACCAA ACTGCAGGACGGGAATACACCTTGTCTGGCAGCTACACCTTCTGA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P06129 UniProtKB Entry Name BTUB_ECOLI GenBank Protein ID 145440 GenBank Gene ID M10112 - General References
- Heller K, Kadner RJ: Nucleotide sequence of the gene for the vitamin B12 receptor protein in the outer membrane of Escherichia coli. J Bacteriol. 1985 Mar;161(3):904-8. [Article]
- Blattner FR, Burland V, Plunkett G 3rd, Sofia HJ, Daniels DL: Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes. Nucleic Acids Res. 1993 Nov 25;21(23):5408-17. [Article]
- Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. [Article]
- Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. [Article]
- Gustafsson C, Lindstrom PH, Hagervall TG, Esberg KB, Bjork GR: The trmA promoter has regulatory features and sequence elements in common with the rRNA P1 promoter family of Escherichia coli. J Bacteriol. 1991 Mar;173(5):1757-64. [Article]
- Link AJ, Robison K, Church GM: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis. 1997 Aug;18(8):1259-313. [Article]
- Dougherty TJ, Thanassi JA, Pucci MJ: The Escherichia coli mutant requiring D-glutamic acid is the result of mutations in two distinct genetic loci. J Bacteriol. 1993 Jan;175(1):111-6. [Article]
- Heller K, Mann BJ, Kadner RJ: Cloning and expression of the gene for the vitamin B12 receptor protein in the outer membrane of Escherichia coli. J Bacteriol. 1985 Mar;161(3):896-903. [Article]
- Gudmundsdottir A, Bell PE, Lundrigan MD, Bradbeer C, Kadner RJ: Point mutations in a conserved region (TonB box) of Escherichia coli outer membrane protein BtuB affect vitamin B12 transport. J Bacteriol. 1989 Dec;171(12):6526-33. [Article]
- Nou X, Kadner RJ: Coupled changes in translation and transcription during cobalamin-dependent regulation of btuB expression in Escherichia coli. J Bacteriol. 1998 Dec;180(24):6719-28. [Article]
- Cadieux N, Kadner RJ: Site-directed disulfide bonding reveals an interaction site between energy-coupling protein TonB and BtuB, the outer membrane cobalamin transporter. Proc Natl Acad Sci U S A. 1999 Sep 14;96(19):10673-8. [Article]
- Coggshall KA, Cadieux N, Piedmont C, Kadner RJ, Cafiso DS: Transport-defective mutations alter the conformation of the energy-coupling motif of an outer membrane transporter. Biochemistry. 2001 Nov 20;40(46):13964-71. [Article]
- Chimento DP, Kadner RJ, Wiener MC: The Escherichia coli outer membrane cobalamin transporter BtuB: structural analysis of calcium and substrate binding, and identification of orthologous transporters by sequence/structure conservation. J Mol Biol. 2003 Oct 3;332(5):999-1014. [Article]
- Chimento DP, Mohanty AK, Kadner RJ, Wiener MC: Crystallization and initial X-ray diffraction of BtuB, the integral membrane cobalamin transporter of Escherichia coli. Acta Crystallogr D Biol Crystallogr. 2003 Mar;59(Pt 3):509-11. Epub 2003 Feb 21. [Article]
- Chimento DP, Mohanty AK, Kadner RJ, Wiener MC: Substrate-induced transmembrane signaling in the cobalamin transporter BtuB. Nat Struct Biol. 2003 May;10(5):394-401. [Article]
- Kurisu G, Zakharov SD, Zhalnina MV, Bano S, Eroukova VY, Rokitskaya TI, Antonenko YN, Wiener MC, Cramer WA: The structure of BtuB with bound colicin E3 R-domain implies a translocon. Nat Struct Biol. 2003 Nov;10(11):948-54. Epub 2003 Oct 5. [Article]