Osmolarity sensor protein EnvZ
Details
- Name
- Osmolarity sensor protein EnvZ
- Synonyms
- 2.7.13.3
- ompB
- perA
- tpo
- Gene Name
- envZ
- Organism
- Escherichia coli (strain K12)
- Amino acid sequence
>lcl|BSEQ0016672|Osmolarity sensor protein EnvZ MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQQFNKVLAYEVRMLMTD KLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWAQHYEFLSHQMAQQLGGPT EVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPLFRYTLAIMLLAIGGAWLFIR IQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTRAFNHMAAGVKQLADDRTLLMAG VSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEECNAIIEQFIDYLRTGQEMPMEMAD LNAVLGEVIAAESGYEREIETALYPGSIEVKMHPLSIKRAVANMVVNAARYGNGWIKVSS GTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVRGDSARTISGTGLGLAIVQRIVDNHNGML ELGTSERGGLSIRAWLPVPVTRAQGTTKEG
- Number of residues
- 450
- Molecular Weight
- 50333.625
- Theoretical pI
- 6.81
- GO Classification
- FunctionsATP binding / phosphoprotein phosphatase activity / phosphorelay sensor kinase activityProcessesdetection of temperature stimulus / peptidyl-histidine phosphorylation / phosphorelay signal transduction system / phosphorylation / protein autophosphorylation / protein dephosphorylation / regulation of response to osmotic stress / signal transduction by protein phosphorylationComponentscytoplasmic side of plasma membrane / integral component of plasma membrane / intracellular
- General Function
- Phosphorelay sensor kinase activity
- Specific Function
- Member of the two-component regulatory system EnvZ/OmpR involved in the regulation of osmoregulation (genes ompF and ompC). EnvZ functions as a membrane-associated protein kinase that phosphorylates OmpR in response to environmental signals.
- Pfam Domain Function
- Transmembrane Regions
- 16-35 159-179
- Cellular Location
- Cell inner membrane
- Gene sequence
>lcl|BSEQ0016673|Osmolarity sensor protein EnvZ (envZ) ATGAGGCGATTGCGCTTCTCGCCACGAAGTTCATTTGCCCGTACGTTATTGCTCATCGTC ACCTTGCTGTTCGCCAGCCTGGTGACGACTTATCTGGTGGTGCTGAACTTCGCGATTTTG CCGAGCCTCCAGCAGTTTAATAAAGTCCTCGCGTACGAAGTGCGTATGTTGATGACCGAC AAACTGCAACTGGAGGACGGCACGCAGTTGGTTGTGCCTCCCGCTTTCCGTCGGGAGATC TACCGTGAGCTGGGGATCTCTCTCTACTCCAACGAGGCTGCCGAAGAGGCAGGTCTGCGT TGGGCGCAACACTATGAATTCTTAAGCCATCAGATGGCGCAGCAACTGGGCGGCCCGACG GAAGTGCGCGTTGAGGTCAACAAAAGTTCGCCTGTCGTCTGGCTGAAAACCTGGCTGTCG CCCAATATCTGGGTACGCGTGCCGCTGACCGAAATTCATCAGGGCGATTTCTCTCCGCTG TTCCGCTATACGCTGGCGATTATGCTATTGGCGATAGGCGGGGCGTGGCTGTTTATTCGT ATCCAGAACCGACCGTTGGTCGATCTCGAACACGCAGCCTTGCAGGTTGGTAAAGGGATT ATTCCGCCGCCGCTGCGTGAGTATGGCGCTTCGGAGGTGCGTTCCGTTACCCGTGCCTTT AACCATATGGCGGCTGGTGTTAAGCAACTGGCGGATGACCGCACGCTGCTGATGGCGGGG GTAAGTCACGACTTGCGCACGCCGCTGACGCGTATTCGCCTGGCGACTGAGATGATGAGC GAGCAGGATGGCTATCTGGCAGAATCGATCAATAAAGATATCGAAGAGTGCAACGCCATC ATTGAGCAGTTTATCGACTACCTGCGCACCGGGCAGGAGATGCCGATGGAAATGGCGGAT CTTAATGCAGTACTCGGTGAGGTGATTGCTGCCGAAAGTGGCTATGAGCGGGAAATTGAA ACCGCGCTTTACCCCGGCAGCATTGAAGTGAAAATGCACCCGCTGTCGATCAAACGCGCG GTGGCGAATATGGTGGTCAACGCCGCCCGTTATGGCAATGGCTGGATCAAAGTCAGCAGC GGAACGGAGCCGAATCGCGCCTGGTTCCAGGTGGAAGATGACGGTCCGGGAATTGCGCCG GAACAACGTAAGCACCTGTTCCAGCCGTTTGTCCGCGGCGACAGTGCGCGCACCATTAGC GGCACGGGATTAGGGCTGGCAATTGTGCAGCGTATCGTGGATAACCATAACGGGATGCTG GAGCTTGGCACCAGCGAGCGGGGCGGGCTTTCCATTCGCGCCTGGCTGCCAGTGCCGGTA ACGCGGGCGCAGGGCACGACAAAAGAAGGGTAA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P0AEJ4 UniProtKB Entry Name ENVZ_ECOLI GenBank Gene ID U18997 - General References
- Mizuno T, Wurtzel ET, Inouye M: Osmoregulation of gene expression. II. DNA sequence of the envZ gene of the ompB operon of Escherichia coli and characterization of its gene product. J Biol Chem. 1982 Nov 25;257(22):13692-8. [Article]
- Comeau DE, Ikenaka K, Tsung KL, Inouye M: Primary characterization of the protein products of the Escherichia coli ompB locus: structure and regulation of synthesis of the OmpR and EnvZ proteins. J Bacteriol. 1985 Nov;164(2):578-84. [Article]
- Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. [Article]
- Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. [Article]
- Roberts DL, Bennett DW, Forst SA: Identification of the site of phosphorylation on the osmosensor, EnvZ, of Escherichia coli. J Biol Chem. 1994 Mar 25;269(12):8728-33. [Article]
- Kanamaru K, Aiba H, Mizuno T: Transmembrane signal transduction and osmoregulation in Escherichia coli: I. Analysis by site-directed mutagenesis of the amino acid residues involved in phosphotransfer between the two regulatory components, EnvZ and OmpR. J Biochem. 1990 Sep;108(3):483-7. [Article]
- Forst S, Comeau D, Norioka S, Inouye M: Localization and membrane topology of EnvZ, a protein involved in osmoregulation of OmpF and OmpC in Escherichia coli. J Biol Chem. 1987 Dec 5;262(34):16433-8. [Article]
- Tokishita S, Kojima A, Mizuno T: Transmembrane signal transduction and osmoregulation in Escherichia coli: functional importance of the transmembrane regions of membrane-located protein kinase, EnvZ. J Biochem. 1992 Jun;111(6):707-13. [Article]
- Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G: Global topology analysis of the Escherichia coli inner membrane proteome. Science. 2005 May 27;308(5726):1321-3. [Article]
- Khorchid A, Inouye M, Ikura M: Structural characterization of Escherichia coli sensor histidine kinase EnvZ: the periplasmic C-terminal core domain is critical for homodimerization. Biochem J. 2005 Jan 1;385(Pt 1):255-64. [Article]
- Kishii R, Falzon L, Yoshida T, Kobayashi H, Inouye M: Structural and functional studies of the HAMP domain of EnvZ, an osmosensing transmembrane histidine kinase in Escherichia coli. J Biol Chem. 2007 Sep 7;282(36):26401-8. Epub 2007 Jul 16. [Article]
- Gerken H, Charlson ES, Cicirelli EM, Kenney LJ, Misra R: MzrA: a novel modulator of the EnvZ/OmpR two-component regulon. Mol Microbiol. 2009 Jun;72(6):1408-22. doi: 10.1111/j.1365-2958.2009.06728.x. Epub 2009 May 8. [Article]
- Gerken H, Misra R: MzrA-EnvZ interactions in the periplasm influence the EnvZ/OmpR two-component regulon. J Bacteriol. 2010 Dec;192(23):6271-8. doi: 10.1128/JB.00855-10. Epub 2010 Oct 1. [Article]
- Tanaka T, Saha SK, Tomomori C, Ishima R, Liu D, Tong KI, Park H, Dutta R, Qin L, Swindells MB, Yamazaki T, Ono AM, Kainosho M, Inouye M, Ikura M: NMR structure of the histidine kinase domain of the E. coli osmosensor EnvZ. Nature. 1998 Nov 5;396(6706):88-92. [Article]
- Tomomori C, Tanaka T, Dutta R, Park H, Saha SK, Zhu Y, Ishima R, Liu D, Tong KI, Kurokawa H, Qian H, Inouye M, Ikura M: Solution structure of the homodimeric core domain of Escherichia coli histidine kinase EnvZ. Nat Struct Biol. 1999 Aug;6(8):729-34. [Article]
- Cai SJ, Khorchid A, Ikura M, Inouye M: Probing catalytically essential domain orientation in histidine kinase EnvZ by targeted disulfide crosslinking. J Mol Biol. 2003 Apr 25;328(2):409-18. [Article]