Aliphatic amidase expression-regulating protein
Details
- Name
- Aliphatic amidase expression-regulating protein
- Synonyms
- Not Available
- Gene Name
- amiC
- Organism
- Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
- Amino acid sequence
>lcl|BSEQ0016430|Aliphatic amidase expression-regulating protein MGSHQERPLIGLLFSETGVTADIERSQRYGALLAVEQLNREGGVGGRPIETLSQDPGGDP DRYRLCAEDFIRNRGVRFLVGCYMSHTRKAVMPVVERADALLCYPTPYEGFEYSPNIVYG GPAPNQNSAPLAAYLIRHYGERVVFIGSDYIYPRESNHVMRHLYRQHGGTVLEEIYIPLY PSDDDVQRAVERIYQARADVVFSTVVGTGTAELYRAIARRYGDGRRPPIASLTTSEAEVA KMESDVAEGQVVVAPYFSSIDTAASRAFVQACHGFFPENATITAWAEAAYWQTLLLGRAA QAAGSWRVEDVQRHLYDICIDAPQGPVRVERQNNHSRLSSRIAEIDARGVFQVRWQSPEP IRPDPYVVVHNLDDWSASMGGGALP
- Number of residues
- 385
- Molecular Weight
- 42806.69
- Theoretical pI
- 5.82
- GO Classification
- Functionsamide binding / kinase activityProcessesamino acid transport / negative regulation of hydrolase activity / regulation of cellular amide catabolic process
- General Function
- Kinase activity
- Specific Function
- Negatively regulates the expression of the aliphatic amidase operon. AmiC functions by inhibiting the action of AmiR at the protein level. It exhibits protein kinase activity.
- Pfam Domain Function
- Not Available
- Transmembrane Regions
- Not Available
- Cellular Location
- Not Available
- Gene sequence
>lcl|BSEQ0016431|Aliphatic amidase expression-regulating protein (amiC) ATGGGATCGCACCAGGAGCGGCCGCTGATCGGCCTGCTGTTCTCCGAAACCGGCGTCACC GCCGATATCGAGCGCTCGCAGCGCTATGGCGCATTGCTTGCGGTCGAGCAACTGAACCGC GAGGGCGGCGTCGGCGGTCGCCCGATCGAAACGCTGTCCCAGGACCCCGGCGGCGATCCG GACCGCTATCGGCTGTGCGCCGAGGACTTCATTCGCAACCGGGGGGTACGGTTCCTCGTG GGCTGCTACATGTCGCACACGCGCAAGGCGGTGATGCCGGTGGTCGAGCGCGCCGACGCG CTGCTCTGCTACCCGACTCCCTACGAGGGCTTCGAGTATTCGCCGAACATCGTCTACGGC GGTCCGGCGCCGAACCAGAACAGTGCGCCGCTGGCGGCGTACCTGATTCGCCACTACGGC GAGCGGGTGGTGTTCATCGGCTCGGACTACATCTATCCGCGGGAAAGCAACCATGTGATG CGCCACCTGTACCGCCAGCACGGCGGCACGGTGCTCGAGGAAATCTACATTCCGCTGTAT CCCTCCGACGACGACGTGCAGCGCGCCGTCGAGCGCATCTACCAGGCGCGCGCCGACGTG GTCTTCTCCACCGTGGTGGGCACCGGCACCGCCGAGCTGTATCGCGCCATCGCCCGTCGC TACGGCGATGGCAGGCGGCCGCCGATCGCCAGCCTGACCACCAGCGAGGCGGAGGTGGCG AAGATGGAGAGCGACGTGGCGGAGGGGCAGGTGGTGGTCGCGCCGTACTTCTCCAGCATC GATACGGCCGCCAGCCGCGCCTTCGTCCAGGCCTGCCATGGCTTCTTCCCGGAAAACGCG ACCATCACCGCCTGGGCCGAGGCGGCCTACTGGCAGACCTTGCTGCTTGGCCGCGCCGCG CAGGCCGCAGGCAGTTGGCGAGTGGAAGACGTGCAGCGGCACCTGTACGACATTTGCATC GACGCGCCCCAGGGGCCGGTCCGGGTGGAGCGCCAGAACAACCACAGCCGCCTGTCTTCG CGCATCGCGGAAATCGATGCGCGCGGCGTGTTCCAGGTCCGCTGGCAGTCGCCCGAACCG ATTCGCCCCGATCCTTATGTCGTCGTGCATAACCTCGACGACTGGTCCGCCAGCATGGGC GGGGGAGCGCTCCCATGA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P27017 UniProtKB Entry Name AMIC_PSEAE GenBank Protein ID 45271 GenBank Gene ID X13776 - General References
- Wilson S, Drew R: Cloning and DNA sequence of amiC, a new gene regulating expression of the Pseudomonas aeruginosa aliphatic amidase, and purification of the amiC product. J Bacteriol. 1991 Aug;173(16):4914-21. [Article]
- Stover CK, Pham XQ, Erwin AL, Mizoguchi SD, Warrener P, Hickey MJ, Brinkman FS, Hufnagle WO, Kowalik DJ, Lagrou M, Garber RL, Goltry L, Tolentino E, Westbrock-Wadman S, Yuan Y, Brody LL, Coulter SN, Folger KR, Kas A, Larbig K, Lim R, Smith K, Spencer D, Wong GK, Wu Z, Paulsen IT, Reizer J, Saier MH, Hancock RE, Lory S, Olson MV: Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen. Nature. 2000 Aug 31;406(6799):959-64. [Article]
- Wilson SA, Chayen NE, Hemmings AM, Drew RE, Pearl LH: Crystallization of and preliminary X-ray data for the negative regulator (AmiC) of the amidase operon of Pseudomonas aeruginosa. J Mol Biol. 1991 Dec 20;222(4):869-71. [Article]
- Pearl L, O'Hara B, Drew R, Wilson S: Crystal structure of AmiC: the controller of transcription antitermination in the amidase operon of Pseudomonas aeruginosa. EMBO J. 1994 Dec 15;13(24):5810-7. [Article]
- O'Hara BP, Norman RA, Wan PT, Roe SM, Barrett TE, Drew RE, Pearl LH: Crystal structure and induction mechanism of AmiC-AmiR: a ligand-regulated transcription antitermination complex. EMBO J. 1999 Oct 1;18(19):5175-86. [Article]
- O'Hara BP, Wilson SA, Lee AW, Roe SM, Siligardi G, Drew RE, Pearl LH: Structural adaptation to selective pressure for altered ligand specificity in the Pseudomonas aeruginosa amide receptor, amiC. Protein Eng. 2000 Feb;13(2):129-32. [Article]