Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro.
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Nagase T, Ishikawa K, Suyama M, Kikuno R, Hirosawa M, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O
Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro.
DNA Res. 1999 Feb 26;6(1):63-70.
- PubMed ID
- 10231032 [ View in PubMed]
- Abstract
As a part of our cDNA project for deducing the coding sequence of unidentified human genes, we newly determined the sequences of 100 cDNA clones from a set of size-fractionated human brain cDNA libraries, and predicted the coding sequences of the corresponding genes, named KIAA0919 to KIAA1018. The sequencing of these clones revealed that the average sizes of the inserts and corresponding open reading frames were 4.9 kb and 2.6 kb (882 amino acid residues), respectively. A computer search of the sequences against the public databases indicated that predicted coding sequences of 87 genes contained sequences similar to known genes, 53% of which (46 genes) were categorized as proteins relating to cell signaling/communication, cell structure/motility and nucleic acid management. The chromosomal locations of the genes were determined by using human-rodent hybrid panels unless their mapping data were already available in the public databases. The expression profiles of all the genes among 10 human tissues, 8 brain regions (amygdala, corpus callosum, cerebellum, caudate nucleus, hippocampus, substania nigra, subthalamic nucleus, and thalamus), spinal cord, fetal brain and fetal liver were also examined by reverse transcription-coupled polymerase chain reaction, products of which were quantified by enzyme-linked immunosorbent assay.
DrugBank Data that Cites this Article
- Polypeptides
Name UniProt ID Protein-arginine deiminase type-2 Q9Y2J8 Details Nischarin Q9Y2I1 Details Calcium/calmodulin-dependent protein kinase type II subunit alpha Q9UQM7 Details Tolloid-like protein 2 Q9Y6L7 Details Serine/threonine-protein kinase 38-like Q9Y2H1 Details Citron Rho-interacting kinase O14578 Details Serine/threonine-protein kinase ICK Q9UPZ9 Details Serine/threonine-protein kinase SIK3 Q9Y2K2 Details Microtubule-associated serine/threonine-protein kinase 1 Q9Y2H9 Details