Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro.

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Citation

Nagase T, Ishikawa K, Suyama M, Kikuno R, Hirosawa M, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O

Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro.

DNA Res. 1999 Feb 26;6(1):63-70.

PubMed ID
10231032 [ View in PubMed
]
Abstract

As a part of our cDNA project for deducing the coding sequence of unidentified human genes, we newly determined the sequences of 100 cDNA clones from a set of size-fractionated human brain cDNA libraries, and predicted the coding sequences of the corresponding genes, named KIAA0919 to KIAA1018. The sequencing of these clones revealed that the average sizes of the inserts and corresponding open reading frames were 4.9 kb and 2.6 kb (882 amino acid residues), respectively. A computer search of the sequences against the public databases indicated that predicted coding sequences of 87 genes contained sequences similar to known genes, 53% of which (46 genes) were categorized as proteins relating to cell signaling/communication, cell structure/motility and nucleic acid management. The chromosomal locations of the genes were determined by using human-rodent hybrid panels unless their mapping data were already available in the public databases. The expression profiles of all the genes among 10 human tissues, 8 brain regions (amygdala, corpus callosum, cerebellum, caudate nucleus, hippocampus, substania nigra, subthalamic nucleus, and thalamus), spinal cord, fetal brain and fetal liver were also examined by reverse transcription-coupled polymerase chain reaction, products of which were quantified by enzyme-linked immunosorbent assay.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Protein-arginine deiminase type-2Q9Y2J8Details
NischarinQ9Y2I1Details
Calcium/calmodulin-dependent protein kinase type II subunit alphaQ9UQM7Details
Tolloid-like protein 2Q9Y6L7Details
Serine/threonine-protein kinase 38-likeQ9Y2H1Details
Citron Rho-interacting kinaseO14578Details
Serine/threonine-protein kinase ICKQ9UPZ9Details
Serine/threonine-protein kinase SIK3Q9Y2K2Details
Microtubule-associated serine/threonine-protein kinase 1Q9Y2H9Details