Human proteasome subunits from 2-dimensional gels identified by partial sequencing.

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Kristensen P, Johnsen AH, Uerkvitz W, Tanaka K, Hendil KB

Human proteasome subunits from 2-dimensional gels identified by partial sequencing.

Biochem Biophys Res Commun. 1994 Dec 30;205(3):1785-9.

PubMed ID
7811265 [ View in PubMed
]
Abstract

Human proteasomes consist of 14 major subunits which can be resolved by two-dimensional polyacrylamide gel electrophoresis. Tryptic peptides from the subunits were sequenced. This allowed identification of all proteins in two-dimensional electrophoresis gels with proteasome subunits whose sequences are known from cDNA. The results also precisely define the specificity of a panel of subunit-specific monoclonal antibodies. A new proteasome subunit (Z) was discovered. In contrast, the putative subunit MECL-1 could not be detected in human placenta proteasomes.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Proteasome subunit beta type-5P28074Details
Proteasome subunit beta type-2P49721Details
Proteasome subunit alpha type-1P25786Details
Proteasome subunit alpha type-2P25787Details
Proteasome subunit alpha type-3P25788Details
Proteasome subunit alpha type-4P25789Details
Proteasome subunit alpha type-6P60900Details
Proteasome subunit beta type-3P49720Details
Proteasome subunit beta type-4P28070Details