Human GM-CSF: molecular cloning of the complementary DNA and purification of the natural and recombinant proteins.
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Wong GG, Witek JS, Temple PA, Wilkens KM, Leary AC, Luxenberg DP, Jones SS, Brown EL, Kay RM, Orr EC, et al.
Human GM-CSF: molecular cloning of the complementary DNA and purification of the natural and recombinant proteins.
Science. 1985 May 17;228(4701):810-5.
- PubMed ID
- 3923623 [ View in PubMed]
- Abstract
Clones of complementary DNA encoding the human lymphokine known as granulocyte-macrophage colony-stimulating factor (GM-CSF) were isolated by means of a mammalian cell (monkey COS cell) expression screening system. One of these clones was used to produce recombinant GM-CSF in mammalian cells. The recombinant hematopoietin was similar to the natural product that was purified to apparent homogeneity from medium conditioned by a human T-cell line. The human T-cell GM-CSF was found to be 60 percent homologous with the GM-CSF recently cloned from murine lung messenger RNA.