Cloning, sequencing and production of the lantibiotic mersacidin.

Article Details

Citation

Bierbaum G, Brotz H, Koller KP, Sahl HG

Cloning, sequencing and production of the lantibiotic mersacidin.

FEMS Microbiol Lett. 1995 Mar 15;127(1-2):121-6.

PubMed ID
7737474 [ View in PubMed
]
Abstract

Mersacidin is a lanthionine-containing peptide antibiotic that shows a good in vivo efficiency against methicillin-resistant Staphylococcus aureus. It is excreted during early stationary phase and could be purified from culture supernatant in a one-step procedure by reversed phase HPLC. Its structural gene was cloned from chromosomal DNA of the producer strain Bacillus subtilis HIL Y-85,54728. Sequencing revealed that pre-mersacidin consists of an unusually long 48 amino acid leader sequence and a 20 amino acid propeptide part which is modified during biosynthesis to the mature lantibiotic. The comparison of the mersacidin prepeptide with those of hitherto known lantibiotics demonstrates that mersacidin is more closely related to type B lantibiotic cinnamycin than to type A lantibiotics.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Lantibiotic mersacidinP43683Details