Nitroreductase-mediated cell ablation in transgenic zebrafish embryos.

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Pisharath H, Parsons MJ

Nitroreductase-mediated cell ablation in transgenic zebrafish embryos.

Methods Mol Biol. 2009;546:133-43. doi: 10.1007/978-1-60327-977-2_9.

PubMed ID

19378102 [ View in PubMed

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Abstract

Prodrug dependent cell ablation is a method that allows inducible and spatially restricted cell destruction. We describe transgenic methods to express the Escherichia coli nfsB in a tissue restricted manner in the zebrafish. This bacterial gene encodes a nitroreductase (NTR) enzyme that can render prodrugs such as metronidazole (Met) cytotoxic. Using the expression of NTR fused to a fluorescent protein, one can simultaneously make cells susceptible to prodrug treatment and visualize cell ablation as it occurs.

DrugBank Data that Cites this Article

Drug Targets

Drug	Target	Kind	Organism	Pharmacological Action	Actions
Metronidazole	Oxygen-insensitive NADPH nitroreductase	Protein	Helicobacter pylori (strain ATCC 700392 / 26695)	Yes	Potentiator	Details