Histamine-induced calcium released from cultured human mucosal microvascular endothelial cells from nasal inferior turbinate.

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Ikeda H, Kubo N, Nakamura A, Harada N, Minamino M, Yamashita T

Histamine-induced calcium released from cultured human mucosal microvascular endothelial cells from nasal inferior turbinate.

Acta Otolaryngol. 1997 Nov;117(6):864-70.

PubMed ID

9442829 [ View in PubMed

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Abstract

Changes in cytosolic Ca2+ concentration ([Ca2+]i) in cultured human mucosal microvascular endothelial cells (HMMECs) from nasal inferior turbinate were measured using a fluorescent Ca(2+)-sensitive dye, fura-2, and photometric fluorescence microscopy. Histamine caused a transient increase in intracellular free Ca2+ in cell populations and in individual cells, followed by a decrease to a sustained elevation. Histamine (100 microM) elevated [Ca2+]i in HMMECs up to 563 +/- 20 nM from a resting level of 60 +/- 45 nM (means +/- SD, n = 31). Promethazine (a histamine H1 receptor antagonist) inhibited [Ca2+]i increase during histamine stimulation, whereas cimetidine (a H2 receptor antagonist) and thioperamide (a H3 receptor antagonist) showed no inhibition. These results suggest that the histamine increase [Ca2+]i in HMMECs induces both a Ca2+ release from stores and a Ca2+ influx through activation of the H1 receptor.

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Drug Targets

Drug	Target	Kind	Organism	Pharmacological Action	Actions
Promethazine	Histamine H1 receptor	Protein	Humans	Yes	Antagonist	Details