16S rRNA mutation associated with tetracycline resistance in a gram-positive bacterium.

Article Details

Citation

Ross JI, Eady EA, Cove JH, Cunliffe WJ

16S rRNA mutation associated with tetracycline resistance in a gram-positive bacterium.

Antimicrob Agents Chemother. 1998 Jul;42(7):1702-5.

PubMed ID
9661007 [ View in PubMed
]
Abstract

A genetic basis for tetracycline resistance in cutaneous propionibacteria was suggested by comparing the nucleotide sequences of the 16S rRNA genes from 16 susceptible and 21 resistant clinical isolates and 6 laboratory-selected tetracycline-resistant mutants of a susceptible strain. Fifteen clinical isolates resistant to tetracycline were found to have cytosine instead of guanine at a position cognate with Escherichia coli 16S rRNA base 1058 in a region important for peptide chain termination and translational accuracy known as helix 34. Cytosine at base 1058 was not detected in the laboratory mutants or the tetracycline-susceptible strains. The apparent mutation was recreated by site-directed mutagenesis in the cloned E. coli ribosomal operon, rrnB, encoded by pKK3535.E. coli strains carrying the mutant plasmid were more resistant to tetracycline than those carrying the wild-type plasmid both in MIC determinations and when grown in tetracycline-containing liquid medium. These data are consistent with a role for the single 16S rRNA base mutation in clinical tetracycline resistance in cutaneous propionibacteria.

DrugBank Data that Cites this Article

Drug Targets
DrugTargetKindOrganismPharmacological ActionActions
Rolitetracycline16S ribosomal RNANucleotideEnteric bacteria and other eubacteria
Yes
Inhibitor
Details