Monitoring aminoglycoside-induced conformational changes in 16S rRNA through acrylamide quenching.

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Chao PW, Chow CS

Monitoring aminoglycoside-induced conformational changes in 16S rRNA through acrylamide quenching.

Bioorg Med Chem. 2007 Jun 1;15(11):3825-31. Epub 2007 Mar 13.

PubMed ID

17399988 [ View in PubMed

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Abstract

Fluorescence of 2-aminopurine (2AP)-substituted A-site and acrylamide quenching were used to study the interactions of paromomycin and neamine with the decoding region of 16S rRNA. The results reveal that paromomycin binding to the A-site RNA leads to increased exposure of residue A1492. In contrast, neamine has little effect on the solvent accessibility of A1492. Electrospray ionization mass spectrometry was used to compare the affinity of paromomycin with the A-site and 2-AP-substituted A-site RNAs.

DrugBank Data that Cites this Article

Drug Targets

Drug	Target	Kind	Organism	Pharmacological Action	Actions
Framycetin	16S ribosomal RNA	Nucleotide	Enteric bacteria and other eubacteria	Yes	Inhibitor	Details
Paromomycin	16S ribosomal RNA	Nucleotide	Enteric bacteria and other eubacteria	Yes	Inhibitor	Details