Biochemical and molecular characterization of tetracycline-resistant Aeromonas veronii isolates from catfish.
Article Details
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Nawaz M, Sung K, Khan SA, Khan AA, Steele R
Biochemical and molecular characterization of tetracycline-resistant Aeromonas veronii isolates from catfish.
Appl Environ Microbiol. 2006 Oct;72(10):6461-6.
- PubMed ID
- 17021193 [ View in PubMed]
- Abstract
Eighty-one tetracycline-resistant Aeromonas sp. strains were isolated from farm-raised catfish. Morphological and biochemical characteristics indicated that 23 of the 81 aeromonads were Aeromonas hydrophila, 7 isolates were Aeromonas trota, 6 isolates were Aeromonas caviae, 42 isolates were Aeromonas veronii, and 3 isolates were Aeromonas jandaei. However, the AluI and MboI restriction fragment length polymorphism (RFLP) patterns of the PCR-amplified 1.4-kb 16S rRNA gene from all 81 tetracycline-resistant aeromonads from catfish were identical to the RFLP banding patterns of A. veronii ATCC 35626, indicating that all 81 isolates were strains of A. veronii. A multiplex PCR assay successfully amplified the 5 tetracycline-resistant genes (tetA to E) from the genomic DNA of all 81 isolates. The assay determined that tetE was the dominant gene occurring in 73/81 (90.0%) of the aeromonads. Plasmids (2.0 to 20 kb) were isolated from 33 of the 81 isolates. Dendrogram analysis of the SpeI pulsed-field gel electrophoresis identified 15 distinct macrorestriction patterns among the isolates. Our results indicate the need for use of 16S rRNA in the identification of Aeromonas spp. and the prevalence of catfish as a reservoir of tet genes.
DrugBank Data that Cites this Article
- Drug Targets
Drug Target Kind Organism Pharmacological Action Actions Chlortetracycline 16S ribosomal RNA Nucleotide Enteric bacteria and other eubacteria YesInhibitorDetails Metacycline 16S ribosomal RNA Nucleotide Enteric bacteria and other eubacteria YesInhibitorDetails Tetracycline 16S ribosomal RNA Nucleotide Enteric bacteria and other eubacteria YesInhibitorDetails