Identification of a soluble form of the angiopoietin receptor TIE-2 released from endothelial cells and present in human blood.
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Reusch P, Barleon B, Weindel K, Martiny-Baron G, Godde A, Siemeister G, Marme D
Identification of a soluble form of the angiopoietin receptor TIE-2 released from endothelial cells and present in human blood.
Angiogenesis. 2001;4(2):123-31.
- PubMed ID
- 11806244 [ View in PubMed]
- Abstract
The transmembrane tyrosine kinase TIE-2, the receptor for the angiopoietins-1 and -2, has been shown to be involved in angiogenic processes. Investigating the regulation of TIE-2 expression on endothelial cells, we found that stimulators such as PMA induce a decrease of TIE-2 protein from the cell surface without affecting TIE-2 mRNA. In conditioned media of PMA stimulated endothelial cells, a soluble form of this receptor comprising parts of the extracellular domain can be detected. Using a sandwich ELISA, we were able to detect and quantify TIE-2 receptors in cell lysates (representing the whole transmembrane receptor) and in cell culture supernatants (representing a soluble form of this receptor, sTIE-2). Several factors influencing the shedding process e.g. basic FGF could be identified. Finally, the soluble form of TIE-2 could also be detected in human biological fluids such as sera and plasma from healthy controls.