Effects of (-)-stepholidine on NMDA receptors: comparison with haloperidol and clozapine.

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Citation

Gu WH, Yang S, Shi WX, Zhen XC, Jin GZ

Effects of (-)-stepholidine on NMDA receptors: comparison with haloperidol and clozapine.

Acta Pharmacol Sin. 2007 Jul;28(7):953-8.

PubMed ID
17588330 [ View in PubMed
]
Abstract

AIM: To examine whether (-)-stepholidine (SPD) has a direct effect on the N-methyl- D-aspartic acid receptors (NMDAR) containing the NMDA receptor subunits NR2A or NR2B and to compare its effect with those of haloperidol (Hal) and clozapine (Cloz). METHODS: NMDAR was transiently expressed in human embryonic kidney 293 (HEK293) cells. Changes in intracellular calcium concentration ([Ca2+]i) induced by NMDAR activation were monitored with Fura-2 ratio imaging techniques. RESULTS: SPD had no significant effects on either subunit of NMDAR at a concentration of less than 100 micromol/L. Hal selectively inhibited NMDAR containing the NR2B subunit, whereas Cloz inhibited both subunits of NMDAR. Although both Hal and Cloz inhibited NR1a/NR2B receptor-mediated Ca2+ influx, their effects were different. Hal was more potent and had a faster peak effect than Cloz. CONCLUSION: Both Hal and Cloz inhibit NMDAR-mediated function, whereas SPD produced only a little inhibition at a high concentration. Based on our other studies, the modulation of SPD on NMDAR function may be via D1 receptor action underlying an indirect mechanism.

DrugBank Data that Cites this Article

Drug Targets
DrugTargetKindOrganismPharmacological ActionActions
HaloperidolGlutamate receptor ionotropic, NMDA 2BProteinHumans
Unknown
Antagonist
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