Mutual displacement interactions in the binding of two drugs to human serum albumin by frontal affinity chromatography.

Article Details

Citation

Nakano NI, Shimamori Y, Yamaguchi S

Mutual displacement interactions in the binding of two drugs to human serum albumin by frontal affinity chromatography.

J Chromatogr. 1980 Feb 1;188(2):347-56.

PubMed ID
7380930 [ View in PubMed
]
Abstract

A continuous frontal analysis chromatographic method was developed for studying the simultaneous binding of two drugs or ligands with an immobilized macromolecule. The usefulness of this method was demonstrated in the interactions of sulphamethizole and salicylic acid with human serum albumin (HSA). The mutual inhibitory effect on the binding of one drug of the presence of the other was directly shown to be due to displacement of the bound drug from HSA by the other. On the basis of a double-reciprocal plot analysis, these two drugs are interpreted as competing for the same primary binding sites.

DrugBank Data that Cites this Article

Drug Carriers
DrugCarrierKindOrganismPharmacological ActionActions
SulfamethizoleSerum albuminProteinHumans
Unknown
Not AvailableDetails