The promoter of the CD19 gene is a target for the B-cell-specific transcription factor BSAP.

Article Details

Citation

Kozmik Z, Wang S, Dorfler P, Adams B, Busslinger M

The promoter of the CD19 gene is a target for the B-cell-specific transcription factor BSAP.

Mol Cell Biol. 1992 Jun;12(6):2662-72.

PubMed ID
1375324 [ View in PubMed
]
Abstract

The CD19 protein is expressed on the surface of all B-lymphoid cells with the exception of terminally differentiated plasma cells and has been implicated as a signal-transducing receptor in the control of proliferation and differentiation. Here we demonstrate complete correlation between the expression pattern of the CD19 gene and the B-cell-specific transcription factor BSAP in a large panel of B-lymphoid cell lines. The human CD19 gene has been cloned, and several BSAP-binding sites have been mapped by in vitro protein-DNA binding studies. In particular, a high-affinity BSAP-binding site instead of a TATA sequence is located in the -30 promoter region upstream of a cluster of heterogeneous transcription start sites. Moreover, this site is occupied by BSAP in vivo in a CD19-expressing B-cell line but not in plasma or HeLa cells. This high-affinity site has been conserved in the promoters of both human and mouse CD19 genes and was furthermore shown to confer B-cell specificity to a beta-globin reporter gene in transient transfection experiments. In addition, BSAP was found to be the only abundant DNA-binding activity of B-cell nuclear extracts that interacts with the CD19 promoter. Together, this evidence strongly implicates BSAP in the regulation of the CD19 gene.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
B-lymphocyte antigen CD19P15391Details