Localization of O-glycosylation sites of MUC1 tandem repeats by QTOF ESI mass spectrometry.

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Citation

Hanisch FG, Green BN, Bateman R, Peter-Katalinic J

Localization of O-glycosylation sites of MUC1 tandem repeats by QTOF ESI mass spectrometry.

J Mass Spectrom. 1998 Apr;33(4):358-62.

PubMed ID
9597769 [ View in PubMed
]
Abstract

The potential of electrospray mass spectrometry (ESMS) for the sequencing of glycopeptides was evaluated using quadrupole time-of-flight (QTOF) technology in the MS/MS mode. The location of O-glycosylation sites was possible in the positive ion (+) mode by detection of prominent y- and b-fragment ions from the underivatized TAP25-2 [T1APPAHGVT9S10APDT14RPAPGS20T21APPA], an overlapping sequence of MUC1 tandem repeats which had been glycosylated in vitro by two GalNAc residues in the positions T9 and T21. The high mass resolution and accuracy of QTOF-(+)ESMS allowed reliable structural assignments. The reduced complexity of the fragment spectra and the higher signal-to-noise ratio render QTOF-(+)ESMS an alternative mass spectrometric approach to the identification of O-glycosylation sites when compared with sequencing by post-source decay matrix-assisted laser desorption/ionization MS. Diagnostic ions from the N-terminus in the b-series offered direct evidence, which was supported by indirect evidence from the C-terminus ions of the y-series. The higher glycosylated GalNAc2-substituted fragments were mainly observed as multiply ionized species.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Mucin-1P15941Details