Isolation and characterization of mammalian HDAC10, a novel histone deacetylase.

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Citation

Kao HY, Lee CH, Komarov A, Han CC, Evans RM

Isolation and characterization of mammalian HDAC10, a novel histone deacetylase.

J Biol Chem. 2002 Jan 4;277(1):187-93. Epub 2001 Oct 24.

PubMed ID
11677242 [ View in PubMed
]
Abstract

Acetylation of histone core particles plays an important role in modulating chromatin structure and gene expression. The acetylation status of the histone tails is determined by two opposing enzymatic activities, histone acetyltransferases and histone deacetylases (HDACs). Here we describe the isolation and characterization of HDAC10, a novel class II histone deacetylase. Molecular cloning and Northern blot analyses reveal that the HDAC10 transcript is widely expressed and subjected to alternative splicing. HDAC10 is both nuclear and cytoplasmic, a feature reminiscent of HDACs 4, 5, and 7. Distinct from other family members, HDAC10 harbors an amino-terminal catalytic domain and a carboxyl pseudo-repeat that shares significant homology with its catalytic domain. Mutational analysis reveals that transcriptional repression by HDAC10 requires its intrinsic histone deacetylase activity. Taken together, HDAC10 represents a distinct HDAC that may play a role in transcription regulation.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Histone deacetylase 10Q969S8Details