Identification of cytochrome P450 1A1 in human brain.
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Yun CH, Park HJ, Kim SJ, Kim HK
Identification of cytochrome P450 1A1 in human brain.
Biochem Biophys Res Commun. 1998 Feb 24;243(3):808-10.
- PubMed ID
- 9500998 [ View in PubMed]
- Abstract
We have isolated cDNA fragments that were originated from P450 1A1 in human brain by polymerase chain reaction (PCR) analysis. Their size matched up to what we expected based on the reported P450 1A1 mRNA sequence of human breast carcinoma cell line MCF-7. To confirm proper PCR products, they were subcloned and their nucleotide sequences were determined. This result showed that PCR products were actually derived from P450 1A1 mRNA in human brain. We demonstrated for the first time that human brain contains a P450 1A1-dependent metabolism system which seems to be important for understanding the metabolism of endogenous and exogenous compounds.