Evaluation of the gene encoding the gamma subunit of rod phosphodiesterase in retinitis pigmentosa.

Article Details

Citation

Hahn LB, Berson EL, Dryja TP

Evaluation of the gene encoding the gamma subunit of rod phosphodiesterase in retinitis pigmentosa.

Invest Ophthalmol Vis Sci. 1994 Mar;35(3):1077-82.

PubMed ID
8125719 [ View in PubMed
]
Abstract

PURPOSE: To determine whether defects in the gene encoding the gamma subunit of rod cyclic guanosine monophosphate-phosphodiesterase (PDE-g) cause some form of hereditary retinal degeneration or dysfunction. METHODS: A restriction map, an intron/exon map, and a partial sequence of the human genomic locus corresponding to this gene were ascertained. Based on this information, the single-strand conformation polymorphism technique (SSCP) was used to screen the coding region as well as most splice donor and acceptor sites for mutations in a total of 704 unrelated patients with retinitis pigmentosa, Usher's syndrome type I or type II, Leber's congenital amaurosis, the Laurence-Moon-Bardet-Biedl syndrome, or other hereditary retinal disease. RESULTS: Two frequent polymorphisms were found, as well as three rare sequence variations, none of which correlated with any phenotype examined. CONCLUSIONS: In view of these negative results and those of a previously published negative Southern blot analysis of an overlapping set of patients, it is unlikely that mutations in the PDE-g gene are a common cause of any of the forms of retinal degeneration or dysfunction so far examined.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gammaP18545Details