Species-dependency in chiral-drug recognition of serum albumin studied by chromatographic methods.

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Fitos I, Visy J, Simonyi M

Species-dependency in chiral-drug recognition of serum albumin studied by chromatographic methods.

J Biochem Biophys Methods. 2002 Dec 31;54(1-3):71-84.

PubMed ID

12543492 [ View in PubMed

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Abstract

Stereoselective binding of benzodiazepine and coumarin drugs to serum albumin from human and six mammalian species were studied by chiral chromatographic techniques. The applied methods were affinity chromatography on the albumins immobilized on Sepharose 4B, high-performance liquid chromatography (HPLC) separation on columns based on human serum albumin (HSA) and bovine serum albumin (BSA), and chiral HPLC analysis of ultrafiltrates of solutions containing the racemic drug and the native protein. Substantial differences in preferred configurations and conformations were detected among the species. The binding stereoselectivity of the 2,3-benzodiazepine drug, tofisopam, in human, is opposite to that in all other species. In the binding of 1,4-benzodiazepines, dog albumin is very similar to HSA. Highly preferred binding of (S)-phenprocoumon was found with dog albumin.

DrugBank Data that Cites this Article

Drug Carriers

Drug	Carrier	Kind	Organism	Pharmacological Action	Actions
Phenprocoumon	Serum albumin	Protein	Humans	No	Binder Regulator	Details