Complete genomic sequence of 195 Kb of human DNA containing the gene GABRG2.

Article Details

Citation

Jiang S, Yu J, Wang J, Tan Z, Xue H, Feng G, He L, Yang H

Complete genomic sequence of 195 Kb of human DNA containing the gene GABRG2.

DNA Seq. 2000;11(5):373-82.

PubMed ID
11328646 [ View in PubMed
]
Abstract

GABA (gamma-aminobutyric acid), as the main inhibitory neurotransmitter in the brain, plays an essential role for the overall balance between neuronal excitation and inhibition by acting on GABAA receptors, which are ligand-gated chloride channels. Impaired GABAergic function contributes to certain forms of epilepsy, schizophrenia, Alzheimer's Disease, and other neurological disorders. In order to identify possible genetic features and to further study biological regulation of GABAA receptor genes whose promoter elements and sequence anomalies may contribute to epileptic disorders, as an initial step, we shot-gun sequenced a BAC clone, dj082c10 (195,909-bp in size), encompassing human gamma(2) subunit of GABAA receptor (GABRG2). It is, we believe, the first genomic sequence of the GABA receptor gamma subunit family. Four contigs were assembled from 2950 reads prior to gap in an average redundancy of eight folds over the entire region. The precision of the consensus sequence was predicted to be 99.999% after closing gaps and finishing weak regions. The nine exons of GABRG2 spans an 85-kb region that had 81 SINEs comprising 22.32%, and nine L1 elements comprising 3.40%, respectively. However, the density of L1 in the regions flanking GABRG2 gene (29.45% by 45 elements) is significantly higher than that within the gene. The length of GABRG2 introns varies in the range of 1.5 kb to 38.1 kb.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Gamma-aminobutyric acid receptor subunit gamma-2P18507Details