Minocycline inhibits the production of inducible nitric oxide synthase in articular chondrocytes.

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Sadowski T, Steinmeyer J

Minocycline inhibits the production of inducible nitric oxide synthase in articular chondrocytes.

J Rheumatol. 2001 Feb;28(2):336-40.

PubMed ID

11246672 [ View in PubMed

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Abstract

OBJECTIVE: To determine the in vitro effects of tetracyclines and nonsteroidal antiiflammatory drugs on interleukin 1alpha (IL-1alpha) induced NO production and biosynthesis of inducible NO synthase (iNOS) by articular chondrocytes. METHODS: Bovine chondrocytes were cultured in alginate beads. Cells were treated with IL-lalpha in the presence or absence of drugs at various concentrations. Expression of mRNA for iNOS was analyzed by reverse transcription polymerase chain reaction-ELISA. Protein synthesis of iNOS was determined by immunoprecipitation. NO production was taken as a measure for the activity of the enzyme. RESULTS: Minocycline dose dependently reduced IL-1 stimulated NO production by inhibition of the mRNA expression (IC50 = 69.9 microM) and protein synthesis (IC50 = 37.11 microM) of iNOS. Diclofenac-Na at a concentration of 10 microM only weakly reduced nitrite accumulation and mRNA expression of iNOS. No effects were observed for tetracycline, doxycycline, and meloxicam. CONCLUSION: Inhibition of iNOS in articular chondrocytes may be a new mechanism by which minocycline could exert its beneficial effects in the treatment of joint diseases.

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Drug Targets

Drug	Target	Kind	Organism	Pharmacological Action	Actions
Minocycline	Interleukin-1 beta	Protein	Humans	Unknown	Modulator	Details