Cloning of a human liver microsomal UDP-glucuronosyltransferase cDNA.
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Jackson MR, McCarthy LR, Harding D, Wilson S, Coughtrie MW, Burchell B
Cloning of a human liver microsomal UDP-glucuronosyltransferase cDNA.
Biochem J. 1987 Mar 1;242(2):581-8.
- PubMed ID
- 3109396 [ View in PubMed]
- Abstract
A cDNA clone (HLUG 25) encoding the complete sequence of a human liver UDP-glucuronosyltransferase was isolated from a lambda gt11 human liver cDNA library. The library was screened by hybridization to a partial-length human UDP-glucuronosyltransferase cDNA (pHUDPGT1) identified from a human liver pEX cDNA expression library by using anti-UDP-glucuronosyltransferase antibodies. The authenticity of the cDNA clone was confirmed by hybrid-select translation and extensive sequence homology to rat liver UDP-glucuronosyltransferase cDNAs. The sequence of HLUG 25 cDNA was determined to be 2104 base-pairs long, including a poly(A) tail, and contains a long open reading frame. The possible site of translation initiation of this sequence is discussed with reference to a rat UDP-glucuronosyltransferase cDNA clone (RLUG 38).