Cloning of human lysozyme gene and expression in the yeast Saccharomyces cerevisiae.
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Castanon MJ, Spevak W, Adolf GR, Chlebowicz-Sledziewska E, Sledziewski A
Cloning of human lysozyme gene and expression in the yeast Saccharomyces cerevisiae.
Gene. 1988 Jun 30;66(2):223-34.
- PubMed ID
- 2971592 [ View in PubMed]
- Abstract
cDNA clones encoding human lysozyme were isolated from a human histiocytic cell line (U-937) and a human placenta cDNA library. The clones, ranging in size from 0.5 to 0.75 kb, were identified by direct hybridization with synthetic oligodeoxynucleotides. The nucleotide sequence coding for the entire protein was determined. The derived amino acid sequence has 100% homology with the published amino acid (aa) sequence; the leader sequence codes for 18 aa. Expression and secretion of human lysozyme in Saccharomyces cerevisiae was achieved by placing the cloned cDNA under the control of a yeast gene promoter (ADH1) and the alpha-factor peptide leader sequence.