Structures of the human and Drosophila 80S ribosome.

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Anger AM, Armache JP, Berninghausen O, Habeck M, Subklewe M, Wilson DN, Beckmann R

Structures of the human and Drosophila 80S ribosome.

Nature. 2013 May 2;497(7447):80-5. doi: 10.1038/nature12104.

PubMed ID
23636399 [ View in PubMed
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Abstract

Protein synthesis in all cells is carried out by macromolecular machines called ribosomes. Although the structures of prokaryotic, yeast and protist ribosomes have been determined, the more complex molecular architecture of metazoan 80S ribosomes has so far remained elusive. Here we present structures of Drosophila melanogaster and Homo sapiens 80S ribosomes in complex with the translation factor eEF2, E-site transfer RNA and Stm1-like proteins, based on high-resolution cryo-electron-microscopy density maps. These structures not only illustrate the co-evolution of metazoan-specific ribosomal RNA with ribosomal proteins but also reveal the presence of two additional structural layers in metazoan ribosomes, a well-ordered inner layer covered by a flexible RNA outer layer. The human and Drosophila ribosome structures will provide the basis for more detailed structural, biochemical and genetic experiments.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Ribosomal protein uL16-likeQ96L21Details
Large ribosomal subunit protein uL13P40429Details
Large ribosomal subunit protein uL14P62829Details
Large ribosomal subunit protein eL15P61313Details
Large ribosomal subunit protein eL19P84098Details
Large ribosomal subunit protein uL23P62750Details
Large ribosomal subunit protein uL2P62917Details
Large ribosomal subunit protein eL37P61927Details
Large ribosomal subunit protein uL3P39023Details
Large ribosomal subunit protein uL5P62913Details
Elongation factor 2P13639Details
Small ribosomal subunit protein uS5P15880Details
Small ribosomal subunit protein RACK1P63244Details
Large ribosomal subunit protein eL14P50914Details
Large ribosomal subunit protein eL18Q07020Details
Large ribosomal subunit protein uL29P42766Details
Large ribosomal subunit protein uL4P36578Details
Small ribosomal subunit protein uS15P62277Details
Small ribosomal subunit protein eS17P08708Details
Small ribosomal subunit protein uS13P62269Details
Small ribosomal subunit protein eS19P39019Details
Small ribosomal subunit protein eS28P62857Details
Small ribosomal subunit protein uS7P46782Details
Small ribosomal subunit protein eS6P62753Details
Small ribosomal subunit protein eS8P62241Details
Small ribosomal subunit protein uS4P46781Details