WD-repeat-propeller-FYVE protein, ProF, binds VAMP2 and protein kinase Czeta.

Article Details

Citation

Fritzius T, Frey AD, Schweneker M, Mayer D, Moelling K

WD-repeat-propeller-FYVE protein, ProF, binds VAMP2 and protein kinase Czeta.

FEBS J. 2007 Mar;274(6):1552-66.

PubMed ID
17313651 [ View in PubMed
]
Abstract

We have recently identified a protein, consisting of seven WD repeats, presumably forming a beta-propeller, and a domain identified in Fab1p, YOTB, VAC1p, and EEA1 (FYVE) domain, ProF. The FYVE domain targets the protein to vesicular membranes, while the WD repeats allow binding of the activated kinases Akt and protein kinase (PK)Czeta. Here, we describe the vesicle-associated membrane protein 2 (VAMP2) as interaction partner of ProF. The interaction is demonstrated with overexpressed and endogenous proteins in mammalian cells. ProF and VAMP2 partially colocalize on vesicular structures with PKCzeta and the proteins form a ternary complex. VAMP2 can be phosphorylated by activated PKCzeta in vitro and the presence of ProF increases the PKCzeta-dependent phosphorylation of VAMP2 in vitro. ProF is an adaptor protein that brings together a kinase with its substrate. VAMP2 is known to regulate docking and fusion of vesicles and to play a role in targeting vesicles to the plasma membrane. The complex may be involved in vesicle cycling in various secretory pathways.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Vesicle-associated membrane protein 2P63027Details
Protein kinase C iota typeP41743Details
Protein kinase C zeta typeQ05513Details