Specific expression of pfkfb4 gene in spermatogonia germ cells and analysis of its 5'-flanking region.
Article Details
- CitationCopy to clipboard
Gomez M, Manzano A, Navarro-Sabate A, Duran J, Obach M, Perales JC, Bartrons R
Specific expression of pfkfb4 gene in spermatogonia germ cells and analysis of its 5'-flanking region.
FEBS Lett. 2005 Jan 17;579(2):357-62.
- PubMed ID
- 15642344 [ View in PubMed]
- Abstract
The results presented demonstrate the expression of pfkfb4 gene in adult testis and in a mouse spermatogonia germ cell line (GC-1spg). The genomic organization of the human pfkfb4 gene shows the existence of 14 exons and 13 introns, spanning 45 kb. A detailed analysis of the 5'-flanking region by transient transfection assays with different 5'-deletion promoter constructs in GC-1spg and mouse sertoli cells (TM-4), allows us to define the minimal promoter unit, containing several GC-rich and ETF sequences along the first -141 nucleotides involved in basal expression. This gene is activated by serum and chemical hypoxia (CoCl(2) treatment) whereas beta-estradiol decreases its expression.