Relation of diltiazem binding sites to opioid receptor subtypes in the guinea-pig brain.

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Citation

Ishizuka Y, Oka T

Relation of diltiazem binding sites to opioid receptor subtypes in the guinea-pig brain.

Tokai J Exp Clin Med. 1987 Mar;12(1):11-7.

PubMed ID
2835832 [ View in PubMed
]
Abstract

Interactions between diltiazem and opioids in guinea-pig brain membrane preparations were examined. Diltiazem did not inhibit bindings of 3H-dihydromorphine (3H-DHM), 3H-ethylketocyclazocine (3H-EKCZ) and 3H-naloxone (3H-NOX) but inhibited binding of 3H-[D-Ala2, D-Leu5]-enkephalin (3H-DADLE). In 3H-DADLE binding, diltiazem decreased the reciprocal indices of affinity (Kd values) of 3H-DADLE without changing on the maximal saturable binding (B. max) in guinea-pig brain membranes. These effects of diltiazem on the binding of 3H-DADLE were enhanced by 2.5mM CaCl2. Morphine, EKCZ, naloxone and even DADLE did not change the binding of 3H-diltiazem. These results suggest that the current of information concerning diltiazem and DADLE binding sites is one-way only so that no interaction exists.

DrugBank Data that Cites this Article

Drug Targets
DrugTargetKindOrganismPharmacological ActionActions
DihydromorphineE3 ubiquitin-protein ligase TRIM13ProteinHumans
Unknown
Agonist
Details