Activation of the c-K-ras oncogene in a human pancreas carcinoma.
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Hirai H, Okabe T, Anraku Y, Fujisawa M, Urabe A, Takaku F
Activation of the c-K-ras oncogene in a human pancreas carcinoma.
Biochem Biophys Res Commun. 1985 Feb 28;127(1):168-74.
- PubMed ID
- 3855240 [ View in PubMed]
- Abstract
The human pancreas carcinoma cell line T3M-4 contains activated c-Kirsten (K)-ras oncogene detectable by the DNA-mediated gene transfer technique using NIH/3T3 cells. DNA fragments containing coding lesions have been cloned, and nucleotide sequence analysis suggests that the T3M-4 oncogene has been activated by a single nucleotide transition from A to C in the second exon, which results in the substitution of histidine for glutamine in coden 61 of the predicted amino acid sequence. The quantity analysis of c-K-ras oncogene in the DNA and RNA of T3M-4 cells revealed that the c-K-ras gene was amplified and overexpressed in T3M-4 cells. These findings indicate that the T3M-4 c-K-ras oncogene is activated by different mutational events.