Isolation and characterization of cDNA clones for the 35-kDa pulmonary surfactant-associated protein.
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Floros J, Steinbrink R, Jacobs K, Phelps D, Kriz R, Recny M, Sultzman L, Jones S, Taeusch HW, Frank HA, et al.
Isolation and characterization of cDNA clones for the 35-kDa pulmonary surfactant-associated protein.
J Biol Chem. 1986 Jul 5;261(19):9029-33.
- PubMed ID
- 3755136 [ View in PubMed]
- Abstract
A group of 35,000-dalton sialoglycoproteins is the major non-serum protein component of pulmonary surfactant. Tryptic fragments of these proteins were sequenced, and oligonucleotide probes were synthesized based on the amino acid sequences. A human lung cDNA library was then screened using the oligonucleotide probes, and clones coding for these proteins were identified and characterized. By in vitro transcription-translation experiments we have associated individual clones with particular proteins. The data suggest that co-translational modifications of two primary translation products account for many of the isoforms observed by two-dimensional gel electrophoresis in the precursors of 35,000-dalton sialoglycoproteins.