Identification of a vitamin K-dependent carboxylase in the venom duct of a Conus snail.

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Stanley TB, Stafford DW, Olivera BM, Bandyopadhyay PK

Identification of a vitamin K-dependent carboxylase in the venom duct of a Conus snail.

FEBS Lett. 1997 Apr 21;407(1):85-8.

PubMed ID

9141486 [ View in PubMed

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Abstract

Peptides from the venom ducts of cone snails (genus Conus) contain gamma-carboxyglutamate residues. The gamma-glutamyl carboxylase responsible for this post-translational modification is localized in the microsomal fraction, strictly dependent on vitamin K, activated by ammonium sulfate, and is associated with endogenous substrate. The K(m) of the enzyme for vitamin K is comparable to that for the bovine carboxylase. However, a propeptide containing substrate related to the blood coagulation protein factor IX, a highly efficient substrate for the bovine enzyme, was poorly carboxylated by the Conus enzyme, suggesting differences in gamma-carboxylase recognition signal sequences and/or structural requirements at the carboxylation site.

DrugBank Data that Cites this Article

Drug Targets

Drug	Target	Kind	Organism	Pharmacological Action	Actions
Coagulation Factor IX (Recombinant)	Vitamin K-dependent gamma-carboxylase	Protein	Humans	Unknown	Not Available	Details
Coagulation Factor IX Human	Vitamin K-dependent gamma-carboxylase	Protein	Humans	Unknown	Not Available	Details