Calorimetric analysis of lisinopril binding to angiotensin I-converting enzyme.

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Tellez-Sanz R, Garcia-Fuentes L, Baron C

Calorimetric analysis of lisinopril binding to angiotensin I-converting enzyme.

FEBS Lett. 1998 Feb 13;423(1):75-80.

PubMed ID

9506845 [ View in PubMed

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Abstract

Isothermal titration microcalorimetry has been used to measure changes in enthalpy and heat capacity for binding of lisinopril to the angiotensin I-converting enzyme (ACE; EC 3.4.15.1) and to its apoenzyme at pH 7.5 over a temperature range of 15-30 degrees C. Calorimetric measurements indicate that lisinopril binds to two sites in the monomer of both holo- and apo-ACE. Binding of lisinopril to both systems is enthalpically unfavorable and, thus, is dominated by a large positive entropy change. The enthalpy change of binding is strongly temperature-dependent for both holo- and apo-ACE, arising from a large heat capacity change of binding equal to -2.4 +/- 0.2 kJ/K/mol of monomeric holo-ACE) and to -1.9 +/- 0.2 kJ/K/mol of monomeric apo-ACE), respectively. The negative values of deltaCp for both systems are consistent with burial of a large non-polar surface area upon binding. Although the binding of lisinopril to holo- and apo-ACE is favored by entropy changes, this is more positive for the holoenzyme. Thus, the interaction between Zn2+ and lisinopril results in a higher affinity of the holoenzyme for this drug due to a more favorable entropic contribution.

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Drug Targets

Drug	Target	Kind	Organism	Pharmacological Action	Actions
Lisinopril	Angiotensin-converting enzyme	Protein	Humans	Yes	Inhibitor	Details