RanBP3 influences interactions between CRM1 and its nuclear protein export substrates.
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Englmeier L, Fornerod M, Bischoff FR, Petosa C, Mattaj IW, Kutay U
RanBP3 influences interactions between CRM1 and its nuclear protein export substrates.
EMBO Rep. 2001 Oct;2(10):926-32. Epub 2001 Sep 24.
- PubMed ID
- 11571268 [ View in PubMed]
- Abstract
We investigated the role of RanBP3, a nuclear member of the Ran-binding protein 1 family, in CRM1-mediated protein export in higher eukaryotes. RanBP3 interacts directly with CRM1 and also forms a trimeric complex with CRM1 and RanGTP. However, RanBP3 does not bind to CRM1 like an export substrate. Instead, it can stabilize CRM1-export substrate interaction. Nuclear RanBP3 stimulates CRM1-dependent protein export in permeabilized cells. These data indicate that RanBP3 functions by a novel mechanism as a cofactor in recognition and export of certain CRM1 substrates. In vitro, RanBP3 binding to CRM1 affects the relative affinity of CRM1 for different substrates.