Potassium channel currents in rat mesenchymal stem cells and their possible roles in cell proliferation.

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Wang SP, Wang JA, Luo RH, Cui WY, Wang H

Potassium channel currents in rat mesenchymal stem cells and their possible roles in cell proliferation.

Clin Exp Pharmacol Physiol. 2008 Sep;35(9):1077-84. doi: 10.1111/j.1440-1681.2008.04964.x. Epub 2008 May 25.

PubMed ID

18505444 [ View in PubMed

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Abstract

Mesenchymal stem cells (MSC) have shown considerable promise for the regeneration and repair of damaged tissue. However, the electrophysiological properties of K+ channels in MSC are not well established and little is known about the role of K+ channels in the regulation of MSC proliferation. We detected three distinct outward currents in MSC: (i) a delayed rectifier current (IK(DR)); (ii) a Ca2+-activated K+ current (IK(Ca)); and (iii) a transient outward K+ current (Ito). All three were present either alone or in combination in almost all cells (90%) investigated. However, 10% of cells did not express a functional current within physiological potentials. Reverse transcription-polymerase chain reaction was used to identify mRNA associated with functional ionic currents. Kv1.2 and Kv2.1 were associated with IK(DR); Slo and KCNN4 were associated with IK(Ca); and Kv1.4 and Kv4.3 were associated with Ito. The Kv channel blockers amiodarone, tetraethylammonium and verapamil, as well as increased extracellular K+ levels, inhibited proliferation of cultured MSC. In MSC treated with Kv channel blockers or an increased extracellular concentration of K+, the proportion of cells in the S phase decreased significantly and the proportion of cells in the G(0)/G(1) phase tended to increase, indicating that the cells were prevented from entering the S phase of the cell cycle. Our findings suggest that rat MSC heterogeneously express distinct types of K+ current, of which the voltage-gated IK(DR)-like K+ current is most common. Kv channel activity modulates the progression of the cell cycle, affecting the proliferation of MSC.

DrugBank Data that Cites this Article

Drug Targets

Drug	Target	Kind	Organism	Pharmacological Action	Actions
Amiodarone	HERG human cardiac K+ channel (Protein Group)	Protein group	Humans	Yes	Inhibitor	Details