Structural basis of Fic-mediated adenylylation.

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Citation

Xiao J, Worby CA, Mattoo S, Sankaran B, Dixon JE

Structural basis of Fic-mediated adenylylation.

Nat Struct Mol Biol. 2010 Aug;17(8):1004-10. doi: 10.1038/nsmb.1867. Epub 2010 Jul 11.

PubMed ID
20622875 [ View in PubMed
]
Abstract

The Fic family of adenylyltransferases, defined by a core HPFx(D/E)GN(G/K)R motif, consists of over 2,700 proteins found in organisms from bacteria to humans. The immunoglobulin-binding protein A (IbpA) from the bacterial pathogen Histophilus somni contains two Fic domains that adenylylate the switch1 tyrosine residue of Rho-family GTPases, allowing the bacteria to subvert host defenses. Here we present the structure of the second Fic domain of IbpA (IbpAFic2) in complex with its substrate, Cdc42. IbpAFic2-bound Cdc42 mimics the GDI-bound state of Rho GTPases, with both its switch1 and switch2 regions gripped by IbpAFic2. Mutations disrupting the IbpAFic2-Cdc42 interface impair adenylylation and cytotoxicity. Notably, the switch1 tyrosine of Cdc42 is adenylylated in the structure, providing the first structural view for this post-translational modification. We also show that the nucleotide-binding mechanism is conserved among Fic proteins and propose a catalytic mechanism for this recently discovered family of enzymes.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Cell division control protein 42 homologP60953Details