Interaction of translation initiation factor 3 with the 30S ribosomal subunit.
Article Details
- CitationCopy to clipboard
Dallas A, Noller HF
Interaction of translation initiation factor 3 with the 30S ribosomal subunit.
Mol Cell. 2001 Oct;8(4):855-64.
- PubMed ID
- 11684020 [ View in PubMed]
- Abstract
Hydroxyl radical footprinting and directed probing from Fe(II)-derivatized IF3 have been used to map the interaction of IF3 relative to 16S rRNA and tRNA(Met)(f) in the 30S ribosomal subunit. Our results place the two domains of IF3 on opposite sides of the initiator tRNA, with the C domain at the platform interface and the N domain at the E site. The C domain coincides with the location of helix 69 of 23S rRNA, explaining the ability of IF3 to block subunit association. The N domain neighbors proteins S7 and S11 and may interfere with E site tRNA binding. Our model suggests that IF3 influences initiator tRNA selection indirectly.