Molecular analysis of 6 desaturase and 6 elongase from Conidiobolus obscurus in the biosynthesis of eicosatetraenoic acid, a omega3 fatty acid with nutraceutical potentials.
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Tan L, Meesapyodsuk D, Qiu X
Molecular analysis of 6 desaturase and 6 elongase from Conidiobolus obscurus in the biosynthesis of eicosatetraenoic acid, a omega3 fatty acid with nutraceutical potentials.
Appl Microbiol Biotechnol. 2011 Apr;90(2):591-601. doi: 10.1007/s00253-010-3060-y. Epub 2011 Jan 6.
- PubMed ID
- 21210105 [ View in PubMed]
- Abstract
Conidiobolus obscurus, an entomopathogenic fungus able to infect aphids, was previously reported to produce substantial amounts of very long chain polyunsaturated fatty acids (VLCPUFAs) that may mediate the insect infection. However, the genes involved in the biosynthesis of these VLCPUFAs from the order Entomophthorales have yet to be identified. Using degenerate reverse transcriptase-polymerase chain reaction and rapid amplification of the cDNA end methods, we cloned a 6 desaturase cDNA (CoD6) and a 6 elongase cDNA (CoE6) from C. obscurus. Expression of CoD6 and CoE6 in Saccharomyces cerevisiae revealed CoD6 could introduce a Delta6 double bond into alpha-linolenic acid (18:3n-3), and CoE6 preferentially elongated 18-carbon Delta6 desaturated fatty acid stearidonic acid (18:4n-3). When the fungus was grown under a temperature shift from 20 degrees C to 10 degrees C, the transcript level of CoD6 and CoE6 increased, whereas when the fungal culture was shifted from 20 degrees C to 30 degrees C, the transcript level of both genes decreased. The entire eicosatetraenoic acid biosynthetic pathway was reconstituted in yeast using four genes, CoD6 and CoE6 from C. obscurus, CpDes12 (a Delta12 desaturase) and CpDesX (a omega3 desaturase) from Claviceps purpurea. Yeast transformants expressing the four genes produced ten new fatty acids including the final product eicosatetraenoic acid (ETA). This represents the reconstitution of the entire ETA pathway in yeast without supplementation of any exogenous fatty acids.
DrugBank Data that Cites this Article
- Drug Targets
Drug Target Kind Organism Pharmacological Action Actions alpha-Linolenic acid Fatty acid desaturase 2 Protein Humans YesLigandDetails