The crystal structure of the PX domain from p40(phox) bound to phosphatidylinositol 3-phosphate.

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Citation

Bravo J, Karathanassis D, Pacold CM, Pacold ME, Ellson CD, Anderson KE, Butler PJ, Lavenir I, Perisic O, Hawkins PT, Stephens L, Williams RL

The crystal structure of the PX domain from p40(phox) bound to phosphatidylinositol 3-phosphate.

Mol Cell. 2001 Oct;8(4):829-39.

PubMed ID
11684018 [ View in PubMed
]
Abstract

More than 50 human proteins with a wide range of functions have a 120 residue phosphoinositide binding module known as the PX domain. The 1.7 A X-ray crystal structure of the PX domain from the p40(phox) subunit of NADPH oxidase bound to PtdIns(3)P shows that the PX domain embraces the 3-phosphate on one side of a water-filled, positively charged pocket and reveals how 3-phosphoinositide specificity is achieved. A chronic granulomatous disease (CGD)-associated mutation in the p47(phox) PX domain that abrogates PtdIns(3)P binding maps to a conserved Arg that does not directly interact with the phosphoinositide but instead appears to stabilize a critical lipid binding loop. The SH3 domain present in the full-length protein does not affect soluble PtdIns(3)P binding to the p40(phox) PX domain.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Neutrophil cytosol factor 4Q15080Details