Identification of a putative isoform of the Na,K-ATPase beta subunit. Primary structure and tissue-specific expression.

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Citation

Martin-Vasallo P, Dackowski W, Emanuel JR, Levenson R

Identification of a putative isoform of the Na,K-ATPase beta subunit. Primary structure and tissue-specific expression.

J Biol Chem. 1989 Mar 15;264(8):4613-8.

PubMed ID
2538450 [ View in PubMed
]
Abstract

We have isolated cDNA clones from rat brain and human liver encoding a putative isoform of the Na,K-ATPase beta subunit. The rat brain cDNA contains an open reading frame of 870 nucleotides coding for a protein of 290 amino acids with a calculated molecular weight of 33,412. The corresponding amino acid sequence shows 98% identity with its human liver counterpart. The proteins encoded by the rat and human cDNAs exhibit a high degree of primary sequence and secondary structure similarity with the rat Na,K-ATPase beta subunit. We have therefore termed the polypeptides these cDNAs encode a beta 2 subunit with the previously characterized rat cDNA encoding a beta 1 subunit. Analysis of rat tissue RNA reveals that the beta 2 subunit gene encodes a 3.4-kilobase mRNA which is expressed in a tissue specific fashion distinct from that of rat beta 1 subunit mRNA. Cell lines derived from the rat central nervous system shown to lack beta 1 subunit mRNA sequences were found to express beta 2 subunit mRNA. These results suggest that different members of the Na,K-ATPase beta subunit family may have specialized functions.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Sodium/potassium-transporting ATPase subunit beta-2P14415Details