Crystallographic studies of Homo sapiens ribosomal decoding A site complexed with aminoglycosides.

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Kondo J, Francois B, Urzhumtsev A, Westhof E

Crystallographic studies of Homo sapiens ribosomal decoding A site complexed with aminoglycosides.

Nucleic Acids Symp Ser (Oxf). 2005;(49):253-4.

PubMed ID

17150729 [ View in PubMed

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Abstract

Aminoglycosides are highly effective antibacterial drugs that decrease translation accuracy by binding to the aminoacyl-tRNA decoding site (A site) of 16S ribosomal RNA. On the other hand, they are highly toxic to mammals through kidney and ear-associated illnesses by binding to ribosomal A sites. To understand the mechanism of toxicity of aminoglycosides to mammals at atomic level, crystallographic studies have been carried out with a number of Homo sapiens mitochondrial and cytoplasmic A sites complexed with aminoglycosides. Several X-ray diffraction data sets were successfully collected. Initial phases of mitochondrial A site with tobramycin and cytoplasmic A site with paromomycin were derived by the molecular replacement method. Refinements of atomic parameters are now under progress.

DrugBank Data that Cites this Article

Drug Targets

Drug	Target	Kind	Organism	Pharmacological Action	Actions
Apramycin	16S ribosomal RNA	Nucleotide	Enteric bacteria and other eubacteria	Unknown	Not Available	Details