p150(Glued), Dynein, and microtubules are specifically required for activation of MKK3/6 and p38 MAPKs.

Article Details

Citation

Cheung PY, Zhang Y, Long J, Lin S, Zhang M, Jiang Y, Wu Z

p150(Glued), Dynein, and microtubules are specifically required for activation of MKK3/6 and p38 MAPKs.

J Biol Chem. 2004 Oct 29;279(44):45308-11. Epub 2004 Sep 16.

PubMed ID
15375157 [ View in PubMed
]
Abstract

To look for regulators of the mitogen-activated protein kinase (MAPK) kinase 6 (MKK6), a yeast two-hybrid screen was initiated using MKK6 as bait. p150(Glued) dynactin, a key component of the cytoplasmic dynein-dynactin motor complex, was found to specifically interact with MKK6 and its close homologue MKK3. Silencing of p150(Glued) expression by small interference RNA reduced the stimulus-induced phosphorylation of MKK3/6 and p38 MAPKs. The similar adverse effect was also seen when the cytoplasmic dynein motor was disrupted by other means. Like p150(Glued), MKK3/6 directly associate with microtubules. Disruption of microtubules prior to cell stimulation specifically inhibits the stimulus-induced phosphorylation of both MKK3/6 and p38 MAPKs. Our unexpected findings reveal a specific requirement for p150(Glued)/dynein/functional microtubules in activation of MKK3/6 and p38 MAPKs in vivo.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Dual specificity mitogen-activated protein kinase kinase 6P52564Details