Molecular cloning, cDNA structure, and chromosomal localization of the human type II cGMP-dependent protein kinase.
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Orstavik S, Solberg R, Tasken K, Nordahl M, Altherr MR, Hansson V, Jahnsen T, Sandberg M
Molecular cloning, cDNA structure, and chromosomal localization of the human type II cGMP-dependent protein kinase.
Biochem Biophys Res Commun. 1996 Mar 27;220(3):759-65.
- PubMed ID
- 8607838 [ View in PubMed]
- Abstract
The type II cGMP-dependent protein kinase is an enzyme originally isolated from the small intestine, and is thought to be involved in the regulation of intestinal ion transport and fluid secretion. A complementary DNA clone encoding a part of the human type II cGMP-dependent protein kinase was isolated from a cerebellum library. Based on sequence information from this complementary DNA, the 5'-end of the type II cGMP-dependent protein kinase was amplified from human brain messenger RNA using polymerase chain reaction. The composite complementary DNA encoded a 762 amino acid protein with a calculated molecular mass of 87.4 kDa. Messenger RNAs encoding the type II cGMP-dependent protein kinase were detected in small intestine, colon and prostate. By using polymerase chain reaction and Southern blotting on somatic cell hybrids, the gene encoding, the type II cGMP-dependent protein kinase was mapped to human chromosome 4q13.1-q21.1.