Cloning of a novel putative protein kinase having a leucine zipper domain from human brain.
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Reddy UR, Pleasure D
Cloning of a novel putative protein kinase having a leucine zipper domain from human brain.
Biochem Biophys Res Commun. 1994 Jul 15;202(1):613-20.
- PubMed ID
- 8037767 [ View in PubMed]
- Abstract
We report a novel putative serine/threonine protein kinase containing a leucine-zipper domain, isolated from an human neuronal cell line. The teratocarcinoma cell line NT2 was differentiated to postmitotic NT2-N neurons by treatment with retinoic acid, and degenerate oligonucleotide primers to the catalytic domains of protein kinases were employed to PCR amplify subtractive cDNAs. We identified a clone, represented at higher abundance in NT2-N neurons than in the parental cell line, which encodes a putative serine/threonine kinase of 859 [corrected] amino acids, the leucine-zipper protein kinase (zpk). Zpk protein contains a leucine-zipper domain, found in many DNA-binding proteins, but few protein kinases. Steady-state mRNA levels for zpk are high in human brain and kidney. Further studies are required to evaluate the role of zpk in neuronal differentiation.