[Study of the interaction between methotrexate and bovine serum albumin by spectrometry].

Article Details

Citation
Copy to clipboard

Xie WJ, Feng YP, Cao SL, Zhao YF

[Study of the interaction between methotrexate and bovine serum albumin by spectrometry].

Guang Pu Xue Yu Guang Pu Fen Xi. 2006 Oct;26(10):1876-9.

PubMed ID
17205742 [ View in PubMed
]
Abstract

The interaction of methotrexate (MTX) and bovine serum albumin (BSA) was studied by fluorescence and absorption spectra. The results show that methotrexate strongly quenched the fluorescence of bovine serum albumin, and the quenching mechanism is a static quenching procedure. The binding constants and the number of binding sites between MTX and BSA at different temperatures were calculated. Furthermore, the enthalpy and entropy changes in the interaction were also obtained, and the primary binding pattern between MTX and BSA was interpreted as hydrophobic interaction.

DrugBank Data that Cites this Article

Drug Carriers
DrugCarrierKindOrganismPharmacological ActionActions
MethotrexateSerum albuminProteinHumans
No
Binder
Details