Binding interaction of gatifloxacin with bovine serum albumin.

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Guo M, Zou JW, Yi PG, Shang ZC, Hu GX, Yu QS

Binding interaction of gatifloxacin with bovine serum albumin.

Anal Sci. 2004 Mar;20(3):465-70.

PubMed ID

15068289 [ View in PubMed

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Abstract

The binding of gatifloxacin to bovine serum albumin (BSA) in aqueous solution was studied using fluorescence spectroscopy and absorbance spectra, Further, the interactions influenced by Fe3+ and Cu2+ were also explored in this work. Based on Scatchard's site-binding model and florescence quenching, practical formulas for small molecule ligands to bio-macromolecules have been proposed. The binding parameters were measured according to suggested models, and the binding distance and the transfer efficiency of energy between gatifloxacin and BSA were also obtained in view of the Forster theory of non-radiation energy transfer. The effect of gatifloxacin on the conformation of BSA has also been analyzed using synchronous fluorescence spectroscopy.

DrugBank Data that Cites this Article

Drug Carriers

Drug	Carrier	Kind	Organism	Pharmacological Action	Actions
Gatifloxacin	Serum albumin	Protein	Humans	Unknown	Other/unknown	Details