Depression of colony formation by human thymus-derived lymphocytes with rifampin and other antimicrobial agents.

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Scharre KA, Eckels DD, Gershwin ME

Depression of colony formation by human thymus-derived lymphocytes with rifampin and other antimicrobial agents.

J Infect Dis. 1981 Jun;143(6):832-5.

PubMed ID

6972981 [ View in PubMed

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Abstract

Colonies of human lymphocytes with thymus-derived (T) cell characteristics can be induced in phytohemagglutinin-P to grow in a semisolid medium. To expand the data base on the effects of antimicrobial agents on cell-mediated immunity, the effect of 30 antimicrobial agents on T-lymphocyte cloning was studied. All of the drugs were added to the cultures in concentrations ranging from 10(-5) to 10(-14) M, and the results were compared with those in cultures without the drug. Drugs that inhibit protein synthesis at the 50S ribosomal subunit in bacteria--in particular, chloramphenicol, clindamycin, erythromycin, and troleandomycin--suppressed colony formation. However, the most significantly immunosuppressive agent was rifampin; it suppressed colony formation at concentrations of up to 2.5 x 10(-9) M, a value significantly lower than that found in previous in vitro testing and well below therapeutic levels. Screening of drugs by lymphocyte cloning techniques for possible suppression of cell-mediated immunity appears to be a rapid, sensitive, and inexpensive procedure.

DrugBank Data that Cites this Article

Drug Targets

Drug	Target	Kind	Organism	Pharmacological Action	Actions
Troleandomycin	50S ribosomal protein L32	Protein	Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)	Yes	Inhibitor	Details
Troleandomycin	50S ribosomal protein L4	Protein	Escherichia coli (strain K12)	Yes	Inhibitor	Details