Ventricular choline transport: a role for organic cation transporter 2 expressed in choroid plexus.
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Sweet DH, Miller DS, Pritchard JB
Ventricular choline transport: a role for organic cation transporter 2 expressed in choroid plexus.
J Biol Chem. 2001 Nov 9;276(45):41611-9. Epub 2001 Sep 11.
- PubMed ID
- 11553644 [ View in PubMed]
- Abstract
To determine whether organic cation transporter (OCT) family members might mediate choline transport in choroid plexus (CP), the handling of choline by cloned transporters and by intact CP isolated from the adult rat was investigated. Expression of OCT1 and OCT2 in Xenopus oocytes increased hemicholinium-3-sensitive choline uptake. In contrast, OCT3 did not mediate choline transport. Estimated K(m) values for choline in rOCT1-, rOCT2-, and hOCT2-expressing oocytes were 346 +/- 50, 441 +/- 67, and 102 +/- 80 microm, respectively. Membrane potential was the major driving force for choline uptake in rat and human OCT2-expressing oocytes and in intact CP in vitro. Lowering of medium pH (6 versus 7.4) was equally effective at inhibiting choline uptake in CP, suggesting that there might be a non-OCT component of choline uptake that is responsive to an H(+) gradient. However, choline efflux from CP was not stimulated by a trans-applied H(+) gradient. Choline uptake by CP was Na(+)-independent with an estimated K(m) of 183 microm. Reverse transcriptase-polymerase chain reaction detected OCT2 and OCT3, but not OCT1, mRNA expression in CP. Transfection of intact CP with a rOCT2/green fluorescent protein fusion construct resulted in strong apical membrane fluorescence with no detectable signal in the basal and lateral plasma membranes. These data indicate that OCT2 mediates choline transport across the ventricular membrane of CP.
DrugBank Data that Cites this Article
- Drug Transporters
Drug Transporter Kind Organism Pharmacological Action Actions Choline Solute carrier family 22 member 1 Protein Humans UnknownSubstrateInhibitorDetails Choline Solute carrier family 22 member 2 Protein Humans UnknownSubstrateInhibitorDetails Choline salicylate Solute carrier family 22 member 1 Protein Humans UnknownSubstrateInhibitorDetails Choline salicylate Solute carrier family 22 member 2 Protein Humans UnknownSubstrateInhibitorDetails Quinine Solute carrier family 22 member 2 Protein Humans UnknownInhibitorDetails