Human organic anion transporters and human organic cation transporters mediate renal transport of prostaglandins.

Article Details

Citation

Kimura H, Takeda M, Narikawa S, Enomoto A, Ichida K, Endou H

Human organic anion transporters and human organic cation transporters mediate renal transport of prostaglandins.

J Pharmacol Exp Ther. 2002 Apr;301(1):293-8.

PubMed ID
11907186 [ View in PubMed
]
Abstract

Prostaglandin E(2) (PGE(2)) and prostaglandin F(2 alpha) (PGF(2 alpha)) have been used for the induction of labor and the termination of pregnancy. Renal excretion is shown to be an important pathway for the elimination of PGE(2) and PGF(2 alpha). The purpose of this study was to elucidate the molecular mechanism of renal PGE(2) and PGF(2 alpha) transport using cells stably expressing human organic anion transporter (hOAT) 1, hOAT2, hOAT3, and hOAT4, and human organic cation transporter (hOCT) 1 and hOCT2. A time- and dose-dependent increase in PGE(2) and PGF(2 alpha) uptake was observed in cells expressing hOAT1, hOAT2, hOAT3, hOAT4, hOCT1, and hOCT2. The K(m) values of PGE(2) uptake by hOAT1, hOAT2, hOAT3, hOAT4, hOCT1, and hOCT2 were 970, 713, 345, 154, 657, and 28.9 nM, respectively, whereas those of PGF(2 alpha) uptake by hOAT1, hOAT3, hOAT4, hOCT1, and hOCT2 were 575, 1092, 692, 477, and 334 nM, respectively. PGE(2) and PGF(2 alpha) significantly inhibited organic anion uptake by hOATs and organic cation uptake by hOCTs. In conclusion, considering the localization of these transporters, the results suggest that PGE(2) and PGF(2 alpha) transport in the basolateral membrane of the proximal tubule is mediated by multiple pathways including hOAT1, hOAT2, hOAT3, and hOCT2, whereas that in the apical side is mediated by hOAT4.

DrugBank Data that Cites this Article

Drug Transporters
DrugTransporterKindOrganismPharmacological ActionActions
Dinoprost tromethamineSolute carrier family 22 member 11ProteinHumans
Unknown
Not AvailableDetails
Dinoprost tromethamineSolute carrier family 22 member 2ProteinHumans
Unknown
Not AvailableDetails
Dinoprost tromethamineSolute carrier family 22 member 6ProteinHumans
Unknown
Not AvailableDetails
Dinoprost tromethamineSolute carrier family 22 member 7ProteinHumans
Unknown
Not AvailableDetails
Dinoprost tromethamineSolute carrier family 22 member 8ProteinHumans
Unknown
Not AvailableDetails
DinoprostoneSolute carrier family 22 member 11ProteinHumans
Unknown
Inhibitor
Details
DinoprostoneSolute carrier family 22 member 2ProteinHumans
Unknown
Inhibitor
Details
DinoprostoneSolute carrier family 22 member 6ProteinHumans
Unknown
Substrate
Inhibitor
Details
DinoprostoneSolute carrier family 22 member 7ProteinHumans
Unknown
Substrate
Inhibitor
Details
DinoprostoneSolute carrier family 22 member 8ProteinHumans
Unknown
Substrate
Inhibitor
Details
LatanoprostSolute carrier family 22 member 6ProteinHumans
Unknown
Substrate
Inhibitor
Details
LatanoprostSolute carrier family 22 member 8ProteinHumans
Unknown
Inhibitor
Details
Polypeptides
NameUniProt ID
Solute carrier family 22 member 7Q9Y694Details